Retrotransfer or gene capture: a feature of conjugative plasmids, with ecological and evolutionary significance.

نویسندگان

  • C Szpirer
  • E Top
  • M Couturier
  • M Mergeay
چکیده

1 Laboratoire de Ge! ne! tique des Prokaryotes, Universite! Libre de Bruxelles, IBMM, B-6041-Gosselies, Belgium 2 Laboratory for Microbial Ecology and Technology, University of Gent, B-9000 Gent, Belgium 3 Environmental Technology, Flemish Institute for Technological Research, VITO, B-2400 Mol, Belgium 4 Laboratory of Microbiology, Radioactive Waste & Clean-up Division, Center of Studies for Nuclear Energy, SCK/CEN, B-2400 Mol, Belgium

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Mechanism of retrotransfer in conjugation: prior transfer of the conjugative plasmid is required.

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Plasmid capture by the Bacillus thuringiensis conjugative plasmid pXO16.

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Plasmids from freshwater environments capable of IncQ retrotransfer are diverse and include pQKH54, a new IncP-1 subgroup archetype.

Nine mercury-resistance plasmids isolated from river epilithon were assessed for their ability to retrotransfer the non-conjugative IncQ plasmid, R300B, derivatives of which have commercial uses that may result in accidental or deliberate release into the environment. Retrotransfer frequencies ranging from 2.1 x 10(-4) to 1.75 x 10(-5) were obtained for five of the nine plasmids--the remaining ...

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Determination of the mechanism of retrotransfer by mechanistic mathematical modeling.

Two mathematical models to elucidate the mechanism of retromobilization (or retrotransfer), that is, the ability of conjugative plasmids to mobilize genes into the cell containing the conjugative plasmid, were developed. This study deals with retromobilization of nonconjugative plasmids (Tra-Mob+). Plasmid transfer was modeled by two mass action models. The first is based on the hypothesis that...

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عنوان ژورنال:
  • Microbiology

دوره 145 ( Pt 12)  شماره 

صفحات  -

تاریخ انتشار 1999